Journal: International Journal of Implant Dentistry
Article Title: Effects of the application of low-temperature atmospheric plasma on titanium implants on wound healing in peri-implant connective tissue in rats
doi: 10.1186/s40729-024-00524-3
Figure Lengend Snippet: Connective tissue attachment. a Expression of adhesion-related genes during wound healing. Relative gene expression levels were quantified based on Day 3 of Cont. ** p < 0.01, * p < 0.05. b Immunohistochemistry of integrin α2, α5, and β1 during wound healing of peri-implant connective tissue. High-magnification histological evaluation during wound healing in peri-implant connective tissue is shown (bars: 20 μm). The regions of interest (ROIs) for integrin localization were set at the peri-implant connective tissue around the apical part of the peri-implant epithelium (shown with H&E staining, bar: 200 μm). Immunoreaction for each integrin was observed in the peri-implant connective tissue
Article Snippet: For primary antibody reaction, sections were incubated with the following primary antibodies for 2 h at room temperature: anti-integrin α2 rabbit polyclonal antibody (1:200; St John’s Laboratory Ltd., London, UK), anti-integrin α5 rabbit polyclonal antibody (1:200; St John’s Laboratory Ltd.), and anti-integrin β1 rabbit polyclonal antibody (1:200; Proteintech Group Inc., Chicago, IL, USA).
Techniques: Expressing, Gene Expression, Immunohistochemistry, Staining